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By Leo A Goldblatt

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Minimum time for aflatoxin production was 2\ days after inoculation. Observations on the relation of time to formation of aflatoxin in peanuts after digging have given varying results. Bampton (1963) pointed out that under tropical conditions (Africa), groundnuts that were free of toxin at digging contained detectable toxin in 48 hours. Also, kernels with testae damaged during shelling showed sporulating A. flavus in 4 days. McDonald and A'Brook (1963) found that 4-6 days in the field before artificial drying gave toxin-free kernels, but samples that had been left for 8-12 days or sun-dried for 10-16 days gave low to medium toxin yields (25-500 j^g/kg).

Miller et al (1957) reported that A. 5% of the isolation plates made in a survey of forest and cultivated soils in northern and southern Georgia. Aspergillusflavushas been frequently reported as a pathogen of man and animals and is considered to be especially important as a pathogen of insects (Austwick, 1965). Chute and Barden (1964) made 42 air samplings over a 12-month period in six chicken hatcheries with an Andersen air sampler and found that A. 3 % of the 10,440 fungus isolates. Aspergillusflavusis generally classified as a saprophyte, although Clinton (1960) has reported post germination and preemergence destruction of peanut seed and seedlings by this fungus in the Sudan.

5°C. 5°C for Early Runner and below 15°C for Florigiant peanuts for a 21-day incubation period (Diener and Davis, 1968a). The upper limiting temperature was slightly above 40°C, since small amounts of aflatoxin were formed at that temperature. Burrell et al. (1964) noted that a constant temperature of 45°C inhibited growth of A. flavus in peanuts and an exposure of 2-4 hours at 50°C checked growth for about 24 hours. Dickens and Pattee (1966) found that in 10 days aflatoxin developed rapidly in peanut samples of 15-30 % moisture held at 90°F (32°C), but in relatively few samples held at 70°F (21 °C).

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