Pathology

Download Bone Marrow Pathology (3rd Edition) by Barbara J. Bain, David M. Clark, Irvin A. Lampert, Bridget PDF

By Barbara J. Bain, David M. Clark, Irvin A. Lampert, Bridget S. Wilkins

Written through one of many world's major haematologists, and 3 well known histopathologists, Bone Marrow Pathology presents a accomplished consultant to the prognosis of bone marrow sickness. Now in its 3rd version, the textual content has been largely revised and rewritten to mirror the most recent advances within the box.
Features:

An super useful, up to date textual content incorporating the recent WHO category of haematopoietic malignancies

A finished textual content written with nice precision and readability of style
Incorporates a brand new part 'Problems and Pitfalls' - a distinct part that might relief the operating pathologist confronted with a tough situation

An vital textual content for the haematologist, histopathologist and haematopathologist with equivalent weight given to peripheral blood, aspirate, trephine biology and really good techniques

Extensively illustrated with the various pictures being of paraffin-embedded sections

Combines all of the innovations now utilized to bone marrow analysis, together with immunocytochemistry, move cytometery, immunohistochemistry and the diagnostic function of cytogenetic and molecular genetic analysis

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Extra info for Bone Marrow Pathology (3rd Edition)

Sample text

Practice differs between laboratories as to whether a Perls’ stain for haemosiderin is performed routinely. If a bone marrow aspirate containing adequate fragments is available, then iron staining of trephine biopsy sections is redundant. However, it may well be more efficient for organizational reasons to perform an iron stain routinely rather than to perform it selectively only in those cases where it is likely to give information of specific diagnostic use. Problems and pitfalls The amount of iron that is leached out when a paraffin-embedded biopsy specimen is decalcified is variable and unpredictable.

Paraffin-embedded, Giemsa ×940. Fig. 7 Section of normal BM: macrophage containing iron. Plastic-embedded, Perls’ stain ×940. 55 56 CHAPTER TWO Fig. 8 Section of trephine biopsy specimen from a child with iron overload associated with congenital sideroblastic anaemia showing a number of plasma cells containing large haemosiderin deposits. Paraffin-embedded, Perls’ stain ×960. in paraffin-embedded bone marrow fragments but not in decalcified trephine biopsy sections. However, no technique for processing and staining of a biopsy specimen allows assessment of whether siderotic granules are normal or decreased; this requires an iron stain of an aspirate.

Fig. 29 Aspirate of normal BM: an immature megakaryocyte with a polyploid nucleus showing little chromatin condensation: the cytoplasm is scanty and basophilic. MGG ×940. The nuclei of the great majority of normal polyploid megakaryocytes form irregular lobes joined by strands of chromatin. A minority have either a non-lobulated nucleus or more than one nucleus. The final stage in megakaryocyte maturation is an apparently bare nucleus (actually with a thin cytoplasmic rim), the great bulk of the cytoplasm having been shed as platelets (Fig.

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